Ацетат диэтиламмония

Identification

Diethylammonium acetate is a salt of diethylamine and acetic acid. English name Diethylammonium acetate, CAS number 20726-63-0, EC number 243-990-1, DSSTox ID DTXSID2066646. Chemical formula C4H11N,C2H4O2 (salt of diethylamine, C4H11N, and acetic acid, C2H4O2), HS code 2921199090. Synonyms: diethylamine acetate, ethanamine N-ethyl acetate, diethylammonium acetate.

Basic Attributes

• Molecular Weight: 133.19
• Exact weight: 133.11000
• PSA specifications: 49.3
• XLogP3: 1.09760

Physico-chemical characteristics

• Melting point: 72–73 °C
• Boiling point: 55.5°C
• Solubility in water: almost clear solution

Substance class

A salt of a secondary amine (diethylamine) and a weak carboxylic acid (acetic acid), belongs to the class of ammonium acetates. Such salts are formed by the reaction of neutralization of an amine with an acid and are characterized by buffering properties in aqueous solutions - the ability to stabilize the pH of the medium in a range close to the pKa of acetic acid.

Scope of application

It is used as a biochemical reagent in proteomic studies, including as a component of buffer systems and mobile phases in chromatographic separation and mass spectrometric analysis of biomolecules, where volatile ammonium salts of organic acids are preferred due to minimal background contamination of the detector. It is also used as a pharmaceutical reagent in laboratory practice.

Benefits of volatile buffers

Unlike classical non-volatile buffer salts (phosphate, borate), diethylammonium acetate completely evaporates during ionization in a mass spectrometer, leaving no mineral deposits on the ion source and without suppressing the signal of target biomolecules. This property is typical of a group of volatile ammonium salts of organic acids used in proteomics and metabolomics as the basis of the mobile phase of liquid chromatography coupled to mass spectrometry.

Storage and transportation conditions

Store in the original tightly closed container, in a cool, dry place, away from sources of moisture and open flame - the substance is hygroscopic and prone to decomposition into its original components (diethylamine and acetic acid) if stored improperly. When working, use respiratory protection and gloves, since diethylamine released during decomposition has a pungent odor and irritant effect.

Temperature comparison

The unusual temperature relationship for an organic salt—the melting point (72–73°C) above the boiling point (55.5°C)—indicates that the boiling point indicated refers to the decomposition of the salt into its volatile components (amine and acetic acid), rather than to the boiling of an individual stable compound. This property is typical for salts of volatile weak acids and bases and should be taken into account when choosing the temperature conditions for storage and processing.

Analytical Compatibility

The volatility of decomposition products (diethylamine and acetic acid) during ionization in the mass spectrometer source is a key operational advantage over classical non-volatile buffer salts: no mineral deposits remain on the ion source and the analytical signal of target peptides and proteins is not suppressed, which determines the choice of such a salt for proteomic and metabolomic methods.