Азометин H

Description

Azomethine H belongs to the class of semicarbazide and sulfonated aromatic Schiff bases. It is the condensation product of salicylic aldehyde with H-acid (4-hydroxy-5-amino-2,7-naphthalenedisulfonic acid). The main use of the substance is as an analytical reagent for the colorimetric and spectrophotometric determination of boron in solutions, soil extracts, plant tissues, water and fertilizers: in the presence of boron, the reagent forms a colored complex with a characteristic absorption maximum.

Basic Attributes

• CAS No.: 32266-60-7
• Chemical formula: C17H13NO8S2
• Molecular Weight: 423.42
• Exact weight: 423.42
• EU number: 250-975-3
• HS code: 2925290090
• PSA specifications: 178.32000
• XLogP3: 4.65660

Physico-chemical characteristics

• Appearance: Crystalline powder
• Density: 1.823 g/cm3
• Melting point: >300 °C
• Solubility: soluble in acetone, ammonium hydroxide, n-butanol and water
• Storage conditions: freezer at -20 °C

Scope of application

Reagent for laboratory and analytical purposes. The main area is agrochemical and soil analysis: determination of mobile boron in soil and solutions using the Azomethine H method, control of boron content in fertilizers and plant materials. It is also used in analytical chemistry as a chromogenic reagent for the quantitative analysis of trace elements by spectrophotometry in the visible region of the spectrum.

Safety Information

• Safety instructions: 24/25
• RTECS number: GV0220000
• Hazard Statements: H315 - Causes skin irritation
• Precautions: P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501
• GHS classification: warning, skin corrosion/irritation

Storage and transportation conditions

Store in a freezer at -20 °C, in a tightly closed container, protected from light and moisture - the substance is sensitive to temperature fluctuations, which is important to consider when transporting in the warm season. When working with the reagent, use gloves and safety glasses, avoid contact with skin and dust on mucous membranes. In case of accidental contact, rinse with plenty of water according to measures P302+P352 and P305+P351+P338. Store separately from food products, in the laboratory - in a specially designated reagent cabinet.

Additionally

The reagent belongs to the group of semicarbazide and naphthalene disulfonic acid derivatives. In solid form it is stable under proper storage conditions, but solutions for colorimetric analysis are prepared immediately before measurement, since the colored complex with boron is sensitive to exposure time and reaction temperature. The Azomethine H method remains one of the most common methods for the quantitative determination of boron in agrochemical laboratories due to the availability of the reagent and the reproducibility of the results.